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Figure 4


Fig. 4. Domains mediating the localisation of AtMinE1. Domains represented as follows: TP, transit peptide; AMD, anti-MinCD domain; hatched box, AtMinD1-interacting domain; TSD, topological specificity domain; red arrow, beta-sheet; red cylinder, {alpha}-helix; CTE, C-terminal extension. For the analysis of N-terminal truncations in living chloroplasts, the transit peptide region from AtABC1 (TP) was fused to AtMinE1117-229 and AtMinE1142-229. The localisation of AtMinE1-YFP, AtMinE11-197-YFP, AtMinE11-169-YFP, AtMinE11-141-CFP, TP.AtMinE1117-229-YFP and TP.AtMinE1142-229-YFP was analysed in tobacco and Arabidopsis by biolistic transformation. Extended-focus images of YFP were captured by epifluorescence microscopy using Volocity II software. Bars, 2 µm.