Fig. 5. Overexpression of AtMinE1 affects chloroplast division. (A) Chloroplast phenotypes in mesophyll and petiole cells of wild-type (WT), 35S-AtMinE1_, 35S-AtMinE1_1-197, 35S-AtMinE1_1-169, 35S-AtMinE1_1-141, 35S-TP.AtMinE1_117-229 and 35S-TP.AtMinE1_142-229 Arabidopsis primary transformants. Extended-focus images of chlorophyll autofluorescence in mesophyll and petiole cells were captured by epifluorescence microscopy using Volocity II software. White arrowheads indicate septation events. Bar, 25 µm. (B) 21-day-old WT and Arabidopsis primary transformants analysed for AtMinE1 and actin expression by RT-PCR. Transcript levels were quantified using imageJ software and the relative amounts of AtMinE1 transcript to actin transcript shown as the means±s.e.m. (with WT=1).