Fig. 7. A model of AtMinE1 interaction and localisation. (A) AtMinE1 interacts with AtMinD1 through one face of an -helix (amino acids 118-140; blue) of the AMD. AtMinE1 dimerisation is mediated through the TSD (amino acids 142-229; red). The minimal region required for dimerisation is amino acids 142-169, although determinants within the rest of the TSD might affect stability. (B) AtMinE1 dimerises through the TSD (red) and interacts with AtMinD1 (yellow) through the AMD (blue). The Min complex localises to one or two spots at the pole of the chloroplast. (C) The AMD alone localises in association with the entire chloroplast inner envelope. (D) The TSD localises to diffuse patches in the chloroplast stroma. AMD, anti-MinCD domain; TSD, topological specificity domain.