Fig. 4. Localization of Dome in mutants with affected intracellular trafficking. (A-D) Immunostaining using anti-Dome antibodies (red) showing Dome localization in Clathrin heavy chain (Chc, A), rab5 (B) and dor (C,D) mutant cells. (A) Mosaic egg chamber containing Chc mutant clones (mutant cells are shown enclosed by a broken line and do not express the GFP clonal marker, green). In Chc mutant clones, Dome is concentrated at the membrane, indicating that Dome is internalized in clathrin-coated vesicles. (B) Mutations in rab5 induce Dome accumulation in follicle cells. Notice the formation of a multi-layered epithelium, as previously observed in follicle cells of rab5 mutant clones (Lu and Bilder, 2005). (C,D) Loss of dor function leads to a strong accumulation of Dome in large intracellular structures corresponding to enlarged multivesicular bodies (MVBs). This phenotype is only seen in dor mutant cells close to the ligand source at the posterior (C) and anterior (D) poles of the egg chamber. Bars, 10 µm.