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Figure 7


Fig. 7. Cdc42 is required for activation of Rac, PI3K and IRSp53 at the protruding edge of EGF-stimulated cells. Control luciferase-siRNA-transfected or Cdc42-siRNA-transfected MTLn3 cells were starved for 3 hours, and stimulated with EGF for 1 or 3 minutes, or not. Representative images of cells immunostained against (A) Rac, (B) PtdIns(3,4,5)P3 or (C) IRSp53. Bar graphs show the average Rac, PtdIns(3,4,5)P3 or IRSp53 fluorescence in the lamellipodia (0.2-0.6 µm from the edge), normalized to edge fluorescence of unstimulated cells. Data are the mean ± s.e.m. from 45 cells. Bars, 10 µm.