Fig. 5. mDia2 is necessary for normal focal adhesion dynamics. (A) Example of focal adhesion dynamics seen in frames from a time-lapse spinning disk confocal microscopy image series of GFP-paxillin in control and anti-mDia2 antibody-injected cells, arrows and arrowheads indicate focal adhesion assembly and disassembly respectively. (B) The average rate constants of FA assembly and disassembly measured from 5-20 focal adhesions per cell in four to five cells per condition (± s.d.). (C) Example of a TIRF-FRAP experiment; GFP-paxillin TIRF images were taken from a time-lapse of a PtK1 cell prior to and after fluorescence photobleaching of the ventral cell cortex, which occurs at t=0. (D) t_1/2 of fluorescent recovery after photobleaching of GFP-paxillin in focal adhesions in control and mDia2 antibody-injected cells, n=5 cells per treatment. (E) Percentage of fluorescent recovery after photobleaching of GFP-paxillin in focal adhesions in control and mDia2 antibody-injected cells.