Fig. 3. Synaptotagmin 3 downregulation by antisense transfection impairs migration. Western blot analysis of SYT3 protein levels in Syt3 transfectants (S, TAM2D2-Syt3), antisense Syt3 transfectants (AS, TAM2D2-anti-sense SYT3), and untransfected cells (T, TAM2D2). After SYT3 staining, blots were stripped and re-probed with actin antibody. Migration assay shows migration towards 100 ng/ml CXCL12, as in Fig. 2B. Data are averages of three experiments. Shown are results shortly after transfection (i.e. after sorting of GFP-high cells and growing of sufficient cells) and 1 month later, in two separate experiments (A and B). Experiments were done with separate sets of cells, transfected on two different occasions.