Fig. 8. TAM2D2 cells, and SYT3-GFP and C2B/SYT3-GFP transfectants were stained with antibodies to (A) EEA1 or both SYT3 and EEA1; (B) M6PR or both SYT3 and M6PR; (C) LAMP1 or both SYT3 and LAMP1, visualized with Cy5-labeled secondary antibodies; or (D) both CXCR4 and LAMP1 and Alexa Fluor 568- and Cy5-labeled secondary antibodies, respectively, and observed by confocal microscopy. (E) CXCR4-GFP and C2B/CXCR4-GFP transfectants were stained with GFP antibody and examined by electron microscopy.