Fig. 1. Distribution and quantitative evaluation of actin isoforms during heart development. (A) 4-µm sections of embryos at E9.5 (a-c), E13 (d-f) and E17 (g-i), and hearts from 2-week-old mice (j-l) were used for immunochemistry with anti
-SMA (a,d,g,j), anti
SKA (b,e,h,k) and anti
-CAA (c,f,i,l) antibodies. Bar, 100 µm. (B) The percentage of
-SMA-positive,
-SKA-positive and
-CAA-positive cardiomyocytes was calculated using the software KS400.