JCS -- Clément et al. 120 (2): 229 Figure IG4

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Fig. 4. Design of shRNAs and their effect on ${alpha}$ -SMA expression in lung fibroblasts (A) Schematic representation of shRNA constructs targeting ${alpha}$ -SMA gene (shSMA1-2-3). A shRNA sequence inactive against all actin isoforms was used as control (shCont). The resulting entry vectors were then recombined into pLenti6/BLOCK-iT^TM RNAi Vector. The loop sequence CGAA is indicated in red. (B) Untransduced mouse lung fibroblasts (a), and mouse lung fibroblasts transduced with shCont (b), shSMA1 (c), shSMA2 (d) and sh-SMA3 (e) were stained for ${alpha}$ -SMA (green). DAPI (blue) was used to stain nuclei. Bar, 20 µm.