Fig. 3. Yap8p stabilization contributes to increased transcriptional activity. An ACR3-promoter-lacZ fusion construct was transformed into wild-type cells and into ubiquitin-proteasome pathway mutants. Transformants were assayed for -galactosidase activity as described in Materials and Methods. The results are the average of three independent experiments and the error bars represent s.d. (A) -galactosidase activity measurements in the pre1-1 pre4-1 mutant (WCG4a background). (B) -galactosidase activity measurements in ubc4 and ubc5 mutants (DF5 background). (C) -galactosidase activity measurements in wild-type cells (WCG4a background) transformed with an empty vector or with a plasmid carrying Yap8p-HA.