Fig. 3. The nuclear distribution of SAFB2 is distinct from that of SAFB1. (A,B). Nuclear distribution of SAFB2 and SAFB1 in HeLa cells. The distribution of SAFB1 protein directly co-localises with the protein detected by monoclonal antibody 6F7, but is distinct from SAFB2. (C) Both SAFB1 and SAFB2 have a much more general subnuclear distribution pattern in HEK293 cells compared with HeLa cells. (D) In adult human testis SAFB2 is specifically upregulated within the nuclei of Sertoli cells (arrowed) whereas SAFB1 expression is negligible in these cells. The images are pseudocoloured, and merged images are shown at the right of the panel. Any overlapping nuclear signal appears yellow. Bars, 10 µm. SC, Sertoli cell; Spg, spermatogonium; Spc, spermatocyte; Rtd, round spermatid.