JCS -- Sergeant et al. 120 (2): 309 Figure IG9

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Fig. 9. SAFB2 and other RNA splicing regulators are quantitatively released into soluble nuclear extracts. To follow the extraction of the different proteins, 1/5000 of several fractions [cytoplasmic fraction (Cyto), pellet 1 (P1), pellet 2 (P2), final nuclear extract (NE)] were analysed by SDS-PAGE followed by Western-blotting using specific antibodies against SAFB1, SAFB2, Sam68, hnRNPG, Tra2 $beta$ and 9G8. Where there are additional aberrantly migrating proteins, the position of the expected protein band is shown with an asterisk.