JCS -- Wang et al. 120 (2): 320 Figure IG5

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Fig. 5. TNF ${alpha}$ induces the death of Cx43-expressing LNCaP cells through TNFR1. (A) Immunoblots showing levels of TNFR1, TNFR2 and $beta$ -actin in uninfected LNCaP cells (Control), LNCaP cells 12 hours after infection with Ad-Cx43 (Ad-Cx43), uninfected LNCaP cells after 12 hours of treatment with 10 ng/ml TNF ${alpha}$ (TNF ${alpha}$ ), and LNCaP cells 12 hours after Ad-Cx43 infection and TNF ${alpha}$ treatment (Ad-Cx43 + TNF ${alpha}$ ). (B) Bar graph showing viability of Cx43-expressing LNCaP cells in the presence or absence of neutralizing anti-TNFR1 (Ab-R1) or –TNFR2 (Ab-R2) antibodies (concentrations indicated in µg/ml). Cell viability determined by MTS assay is expressed as a percentage of control values. Statistical analysis of the raw absorbance data showed that treatment with 1 or 10 µg/ml of anti-TNFR1 antibodies or the combination of 10 µg/ml anti-TNFR1 and 10 µg/ml anti-TNFR2 antibodies significantly antagonized the effects of TNF ${alpha}$ (P<0.0001 when compared to the viability of cells treated with TNF ${alpha}$ alone).