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Figure 5


Fig. 5. BAD-LAMP organization is defined by microtubules. Immunofluorescence confocal microscopy of cortical neurons. (A) BAD-LAMP vesicles (red) accumulate in areas of the cell that are strongly enriched in the phospho-epitope detected by the Smi31 antibody (blue), whereas the L1 molecule (green) is distributed throughout the neuronal plasma membrane. (B) (top) BAD-LAMP vesicle accumulation (white) coincides with microtubule bundling (beta-tubulin, red) and weak GM1 staining (green). Higher magnification (Z1) shows that BAD-LAMP vesicles align along microtubules. (Bottom) Nocodazole treatment induces microtubule destabilization and disorganization of BAD-LAMP vesicle clusters. Bars, 20 µm; 10 µm for Z1.