Fig. 8. Localization of FLAG-tagged BAD-LAMP in transfected cortical neurons. Cortical neurons co-transfected with BAD-GFP and FLAG-BAD-LAMP were visualized by confocal microscopy. (A) Staining for FLAG antibody (red), BAD-GFP (green) and LAMP2 (white). FLAG-BAD-LAMP does not co-localize with LAMP 2. BAD-GFP is targeted to lysosomes upon addition of the GFP moiety at the C-terminal end of BAD-LAMP. Bar, 20 µm; 10 µm for high magnification of Z1 and 5 µm for Z2. (B) Internalization of FLAG antibody (red) in transfected neurons for indicated times and staining for LAMP2 (white). High magnifications reveal a late accessibility of BAD-LAMP into LAMP2 positive compartments in neurites. Bars, 20 µm; 5 µm for high magnification.