Fig. 8. Tfn does not accumulate at the uropod membrane in AP-2 blocked cells. (A) ICAM-3 spontaneously clusters at the uropod tip (green) of both a DIII+ (cyan) and a non-transfected cell. Internalized Tfn only appears in the non-transfected cell (red). The image is a z projection, and the DIC image merged with ICAM-3 labeling is inserted. (B) Time-lapse projections of Tfn-Tx dynamics in a DIII+ (arrow) and a control cell, as indicated. Both cells are polarized (F, front; U, uropod) and representative (10/10). Note that Tfn only enters the control cell, and that it remains evenly distributed on the membrane of the DIII+ cell, with no apparent redistribution towards the rear part along time (see C). (C) Intensity profiles from the front to the rear membrane (blue lines) at different Tfn-chase times (1, 2, 5 min). Note that Tfn is homogenously distributed at the first minute in both cells, and eventually redistributes towards the uropod neck of the control cell (arrowheads). This usual accumulation was never observed in AP-2-blocked cells (DIII+). DIC images merged to Tfn (red) or DIII-GFP (green), are shown at 5 minutes.