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Figure 1


Fig. 1. Characterization of MDCK ADAMTS12 stable transfectants. (A) Domain organization of ADAMTS12 showing the position of the FLAG epitope. (B) RT-PCR and western blot analysis of the ADAMTS12-expressing clones TS12-4 and TS12-22, using the H-142 antibody against ADAMTS12. The cMDCK clone was used as a negative control in these assays. In RT-PCR experiments, amplification of mRNA encoding beta-actin was used to ascertain RNA integrity and ensure equal loading. (C) Immunostaining of non-permeabilized cMDCK, TS12-4 and TS12-22 cells using an anti-FLAG antibody. Bar, 10 µm.