Fig. 7. Effect of ADAMTS12 on tubulogenesis of BAE-1 cells. BAE-1 cells were cultured within a collagen gel for 2 days in the absence (a) or presence (b–f) of 100 ng/ml VEGF. 10 µl of the cell-layer-associated fraction from 293-EBNA cells that express ADAMTS12 (EBNA-TS12) (c), or the same amount of the equivalent fraction from c-EBNA cells (d), were added to the culture. BAE-1 cells were grown in the presence of 10 µl of purified ADAMTS12 (e), or the same amount of the equivalent fraction purified from c-EBNA cells (f). Addition of 50 µl of conditioned medium from cells that express truncated ADAMTS12 (g) or from control cells transfected with an empty vector (h) does not affect the ability of BAE-1 cells to form a capillary network in the presence of VEGF. Bar, 30 µm.