Fig. 6. Relationship of 3F3A-labelled SER tubules to other ER markers upon ATP-induced mitochondrial dissociation. MDCK cells untreated (A,B,E,F) or treated for 2 minutes with 10 µM ATP (C,D,G,H) were fixed and double immunofluorescently labelled for 3F3A and calnexin (A-D) or for 3F3A and SERCA (E-H). Merged images show 3F3A (red) and calnexin (green) co-distribution (A'-C') or 3F3A (red) and SERCA (green) co-distribution (E'-G') in yellow. A'', C'', E'' and G'' show details of the boxed regions from images A', C', E' and G', respectively. Bars, 20 µm (G'); 4 µm (G'').