Fig. 2. MCPH1 cellular localisation in the syncytial embryo varies through the cell cycle. (A) Localisation of endogenous MCPH1 during phases of the cell cycle in syncytial blastoderm embryos. DNA (blue, DAPI); affinity purified anti-MCPH1 antibody (green). MCPH1 is detectable in prophase (Pro) and interphase (Inter) nuclei and is not detectable in prometaphase (Prometa), metaphase (Meta), anaphase (Ana) or telophase (Telo). Bar, 5 µm. (B) Microcephalin is expressed at constant levels throughout mitosis. Methanol fixed, DAPI stained embryos from cycles 8-10 were hand sorted, and 15 embryos per mitotic stage were analysed by SDS-PAGE and immunoblotting with anti-MCPH1 antibody.