Fig. 3. Sds21.NEGFP accumulates in nuclei, dots within the nucleus and at the cell periphery. Cells of the indicated strains were grown as for Fig. 1 with (A-C) or without (D,E) 10 µg/ml Hoechst 33342. In D and E the strains indicated in the central panel were transiently exposed to TRITC-lectin before being mixed and mounted with the deletion strain. (A,B) Sds21.NEGFP accumulates in nucleoli of dis2⁺ cells (IH2352) and is recruited to all sites normally occupied by Dis2.NEGFP when the dis2⁺ gene has been deleted (IH2635). (C) The distribution of Dis2.NEGFP is not affected by deletion of sds21⁺(IH2090). (D) Imaging sds21.NEGFP cells that have been dipped in red lectin (central micrograph) alongside sds21.NEGFP dis2. cells shows an increase in the intensity of the Sds21.NEGFP signal upon deletion of dis2⁺. (E) Imaging dis2.NEGFP cells that have been dipped in red lectin (central micrograph) alongside dis2.NEGFP sds21. cells shows no increase in the intensity of the Dis2.NEGFP signal upon deletion of sds21⁺. (F) Western blotting with antibodies to GFP establishes that the increase in Sds21.NEGFP signal intensity in dis2. arises from an increase in protein levels. A-E. Bar, 5 µm.