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Figure 7


Fig. 7. Abolishing Tea1, Tea2 and Mal3 function abolishes Dis2.NEGFP tip association. Cells of the indicated strains were processed as described for Fig. 1. (A-C) Deletion of pom1+(IH2349; A), bud6+ (IH3092; B) or tea3+ (IH3202; C) did not affect Dis2.NEGFP association with the cell tips. (D-G) Deletion of tea1+ (IH3201; E), mal3+ (IH3155; F) or tip1+ (IH3757; G), and the presence of tea2.1 (IH2859) grown at 36°C (D) abolished the association of Dis2.NEGFP with cell tips. (H) Quantification of Dis2.NEGFP cap formation. Bars, 5 µm.