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Figure 8


Fig. 8. Transit through NETO and Tea1 function control DEAF distribution. (A) dis2.NEGFP tea1{Delta} cells (IH3210) were processed as in Fig. 6A. Deletion of tea1+ resulted in a polarised distribution of DEAFs towards the tip, at which new cell wall had been added above the TRITC-lectin coating, indicating that it was the growing tip. Bar, 5 µm. (B) Quantification of DEAF distribution in the indicated strains shows that Tea1 is required to ensure that endocytosis occurs to a similar degree on both sides of the interphase nucleus. The figures on the y axis are a ratio of the number of dots seen on one side of the nucleus against those on the other.