Fig. 4. Actin cytoskeleton rearrangement does not occur during incomplete cytokinesis. (A) Hepatocytes were isolated from either 15- or 25-day-old rats. Hepatocytes were stained with Alexa-Fluor-488-phalloidin (Actin) and nuclei with Hoechst (DNA). A total of 200 cells from four independent experiments were analyzed. (B) The Alexa-Fluor-488-phalloidin fluorescence profile was determined along the cortex in early telophase of complete (left) and incomplete (right) cytokinesis. Quantification was measured only on half of the cell, from one pole to the other (see upper scheme, red line). The broken vertical lines surround the equatorial area. A total of 50 early telophase cells were analyzed in four independent cultures. (C) Cell elongation from metaphase to telophase was determined using time-lapse sequences; hepatocytes were treated or not with HA1077 (15-day-old rats). Time 0 correspond to chromosome alignment along the metaphase plate. Data are presented as the mean±s.e. from ten independent time-lapse sequences. (D) Hepatocytes were isolated as in A. Hepatocytes were stained with anti-phospho-Myosin light chain 2 (Phospho-RLC) and nuclei with Hoechst (DNA). Early telophase cells are shown (n=200 from four independent cultures). Bars, 5 µm.