Fig. 5. Organization of astral and central spindle microtubules during incomplete cytokinesis. (A) Hepatocytes were isolated from either 15- or 25-day-old rats. Hepatocytes were stained with anti--tubulin and nuclei with Hoechst (DNA). A total of 200 cells from four independent experiments were analyzed. Bars, 5 µm. (B,C) Hepatocytes (25-day-old rats) were stained with anti--tubulin (B, microtubules, green) or anti-EB1 (C, microtubule tips, green) and anti--catenin (B,C, cell cortex, red). A total of 100 cells from four independent experiments were analyzed. (B) Images represent magnified equatorial regions of anaphase hepatocytes. Bars, 1 µm. (C) Nuclei were stained with Hoechst (blue). Boxed areas are shown below: higher-magnification of the equatorial cortical region. Bars, 5 µm.