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Figure 10


Fig. 10. Localization of hNPC2 is dependent on the AP-3 complex. Equal numbers of AP-3+/+ and AP-3–/– mouse fibroblasts were infected with adenovirus containing hNPC2-GFP (top set) or, as a control, GFP alone (top set). Media and cell lysates were collected for immunoblot analysis. As a control for cell type and loading, blots were analyzed for the presence of the AP-3 {delta} subunit and tubulin, respectively. Samples are shown in duplicate.