Fig. 3. The AP-2 complex is not required for the localization of scNcr1p or scNpc2p. GFP-ALP (top), scNCR1-GFP (middle) and scNPC2-GFP (bottom) plasmids were transformed into a yeast deletion of the AP-2 complex (apl1
). The GFP-tagged proteins were visualized by direct fluorescence microscopy. The corresponding DIC images are shown.