Fig. 6. Quantification of the co-distribution of mNPC1 and hNPC2 with various organellar markers. All images were quantified using Metamorph software. Values from AP-3^+/+ cells are denoted with white bars and those from AP-3^–/– cells with gray bars. All results are the average of 10-20 quantified images, except for adaptor co-localizations, for which 30 images were analyzed. The results from one of three independent experiments are depicted. Standard deviations are indicated. (A) Percentages of co-localization of mNPC1 with AP-3, AP-1, LAMPI, syntaxin 8 (Syn8), Vti1b or transferrin receptor (TfR) in AP-3^+/+ and AP-3^–/– cells. P values are ^*1 and ^*2 P<0.0001. (B) Percentages of co-localization of mNPC1 with Vti1b and Syn8 in freshly isolated primary skin fibroblasts from grizzled and mocha mice. P values are ^*1 P<0.001 and ^*2 P<0.0001. (C) Percentages of co-localization of LAMPI with Vti1b and Syn8 in AP-3^+/+ and AP-3^–/– cells. P values are ^*1 P<0.02 and ^*2 P<0.03. (D) Percentages of co-localization of hNPC2 with AP-3, AP-1, LAMPI, Syn8, Vti1b and TfR in AP-3^+/+ and AP-3^–/– cells.