Fig. 4. FAP133 is present in strains lacking axonemal substructures. Flagella from wild-type Chlamydomonas (CC124) and strains lacking various axonemal substructures including the outer dynein arm alone (oda6) or in combination with the docking complex (oda3) or Oda5p-adenylate kinase complex (oda5), inner dynein arm I1/f (ida1), inner arms a, c and d (ida4), the radial spokes (pf14) and central pair microtubule complex (pf18) were electrophoresed in a 5-15% polyacrylamide gradient gel and stained with Coomassie Blue (upper panel) or blotted and probed with antibody against FAP133 (lower panel). All mutant strains contain essentially wild-type levels of FAP133.