Fig. 8. FAP133 associates with LC8. Flagellar matrix components were fractionated using a Mono-Q anion-exchange column. The eluted fractions were separated in a 5-15% polyacrylamide gel and either stained with Coomassie Blue (upper panel) or transferred to a nitrocellulose membrane and probed for FAP133 (middle panel). The fractions that contained the two FAP133 peaks were pooled and further analyzed in two 5 ml 5-20% sucrose density gradients. Immunoblots of both gradients (lower left and right panel pairs) were probed for FAP133 and LC8. In both gradients FAP133 and LC8 were located in the same fractions at 10 S.