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Figure 2


Fig. 2. Sparse-labeled monolayer cell migration assay. Fluorescently labeled cells were plated 1:10 with unlabeled cells to form a confluent monolayer in a 96-well plate. Images were acquired at 15-minute intervals for 6-8 hours using a Cellomics KineticScan. Labeled cells within the monolayer were tracked using Imaris. Cell paths from a sample field for a single condition are shown. Individual cell paths are displayed as a function of time.