Fig. 1. Analysis pipeline. An example of the analysis pipeline, used in an RNAi screen of Drosophila spindle morphology. After acquisition, the images were streamlined to the image database. The analysis code accessed the raw images, segmented them to identify cells and classified the cells to distinguish mitotic cells from apoptotic and interphase cells. The mitotic cells were reorganized into galleries for visual analysis. The same cells from the galleries were analyzed further to identify specific mitotic phenotypes. Bootstrap statistics was used to identify wells that exhibited statistically significant extreme phenotypes.