Fig. 4. Depletion of RILP retards internalized EGFRs in early endosomes. HeLa cells transfected with control RNA or RILP siRNA were pretreated with 10 µg/ml cycloheximide (1 hour) and stimulated with 50 ng/ml EGF for 2 hours. The cells were permeabilized before fixation and stained with antibodies against EGFR (red), Lamp1 (green) and EEA1 (blue).