Fig. 7. Increased LBPA and Lamp1 staining in RILP-depleted cells. (A) HeLa cells treated with control RNA (upper panels) or siRNA against RILP (lower panels) were fixed and stained with antibodies against LBPA (red), Lamp1 (green) and EEA1 (blue) and studied by confocal microscopy under identical settings. Merged images are shown in the right-hand panels. Note the increased staining of LBPA and Lamp1 in the RILP-depleted cells. (B) Quantification of results from confocal microscopy (20 cells from two different experiments) showed significantly increased LBPA and Lamp1 intensities in RILP-depleted cells compared with that of control cells (P<0.05 according to Student's t-test).