Fig. 6. RANBP1-depleted mitoses harbour hyperstable MTs. (A) Graph represents the frequency of mitotic cells with resistant MTs (MTs shown in green in images on right) after 12 or 20 hours of incubation in 50 ng/ml NOC in RANBP1-interfered [RANBP1(i)] cultures and in controls interfered with non-specific siRNAs. Examples of mitotic cells at similar stages that did assemble MTs (indicated as resistant, a) or displaying depolymerized MTs (b,c) are shown on the right. A total of 300 mitotic cells were examined in two independent experiments. ^*Differences between RANBP1-depleted and controls cells were statistically significant (P<0.01) using the ² test. (B) Cold-resistant MTs in GL2- and RANBP1-interfered cells. Representative MT phenotypes are shown (-tubulin, green; CREST, red); the frequency of each phenotype is quantified in the table below. Statistical differences were calculated using the ² test. ns, non significant. Bars, 10 µm.