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Figure 7


Fig. 7. RANBP1-interfered cells show chromosome segregation defects. (A) Examples of mitotic abnormalities (arrows) in RANBP1-interfered [RANBP1(i)] U20S cells. Bar, 10 µm. (B) Time-course analysis of mitotic abnormalities. The efficiency of RANBP1-interference during synchronization was monitored by western blot. We began to collect mitotic cells 9 hours after release from G1/S arrest; thereafter, samples were harvested every 20 minutes and were examined for mitotic abnormalities. Graph shows the frequency of abnormalities at the indicated times; at least 300 mitotic cells were counted for each time point in two experiments. thym, thymidine; meta, metaphase; ana, anaphase; telo, telophase. (C) Representative metaphase (a-d) showing CREST-stained KTs (red); the left panels show merged pictures with MTs ({alpha}-tubulin, green). Anaphase images (e-j) show extending MTs between separating chromosomes in RANBP1-(h-j) but not in GL2-(e-g) interfered cells.