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Figure 3


Fig. 3. Microtubule depolymerization causes dispersion of EYFP-STIM1 throughout the ER. (A) Confocal images of HEK 293 cells co-overexpressing EYFP-STIM1 (left panel; green) and an ER-targeted CFP (CFP-ER) (middle panel; red) in the presence of 1.8 mM extracellular Ca2+ (upper panel) and following a 20 minute treatment with nocodazole (NZL; 10 µM; lower panel). (B) Images of HEK 293 cells expressing EYFP-STIM1 that were treated with nocodazole for 20 minutes, followed by fixation and immunostaining with antibodies against GFP (left panel; green) and {alpha}-tubulin (middle panel; red). Bars, 10 µm.