Fig. 3. Antibody specificity and Panx1 and Panx3 species. Affinity-purified anti-Panx1 antibody shows high specificity to Panx1 both in immunolabeling (A) and western blot (B) when pre-adsorbed with cognate peptides. (B) Multiple species of Panx1 at 41-48 kD and Panx1-GFP at 70-80 kD were detected. (C,D) Affinity-purified anti-Panx3 antibody revealed high specificity to Panx3 both in immunolabeling (C) and western blot (D) when pre-absorbed with cognate peptides. Only one species of Panx3 or Panx3-GFP was detected at 43 kD or 70 kD, respectively. Anti-GAPDH was used to assess gel loading. Bars, 10 µm.