Fig. 4. Unlike Cx43-GFP, cell surface Panx1 and Panx3 localization is unaffected by BFA treatment. BICR-M1R_k cells co-expressing Cx43-GFP and Panx1 (A) or Panx3 (B) were treated with 5 µg/ml BFA for 6 hours. After treatment, Cx43-GFP plaques internalized from the cell surface and newly biosynthesized Cx43-GFP accumulated within intracellular compartments, whereas the localization of Panx1 and Panx3 remained unchanged. The experiment shown is representative of three independent repeats. Bars, 20 µm.