Fig. 5. Panx1 expression levels were unchanged during cycloheximide treatment. BICR-M1R_k cells expressing endogenous Cx43 and exogenous Panx1 were exposed to 20 µg/ml of CHX for 2, 4, 6 and 8 hours and immunoblotted for Panx1 (A) and Cx43 (B). When compared to un-treated cells (0 hr), immunoblot analysis of Panx1 revealed no significant difference (P=0.4980) between the banding profile at 2, 4, 6 and 8 hours of CHX treatment. However, given the known short half-life of Cx43, the level of Cx43 consistently diminished following 2, 4, 6 and 8 hours of CHX treatment. -actin was used as a protein loading control. The experiment shown is representative of three independent repeats. WT, wild type.