Fig. 8. Tissue expression of Panx1 and Panx3. Affinity-purified anti-Panx1 (A) and anti-Panx3 (B,C) antibodies were used to examine the expression of Panx1 and Panx3 in several tissue types from 3-week-old mice and in the thymus from neonatal mice. (A) Panx1 was prevalent in many murine organs and exhibited variable degrees of apparent glycosylation (41-48 kD), whereas two putative isoforms of Panx3 (43 kD and 70 kD) were observed in skin, cartilage and heart ventricle (B). (C) Only a 70 kD band was detected with the anti-Panx3 antibody in lung, liver, spleen and thymus, with a notable 50 kD band present in kidney, whereas brain tissue exhibited little or no detectable Panx3. The tissues and Panx3 control used in C were obtained from the same gel. A peptide pre-adsorption assay eliminated most of the staining both for the Panx1 and Panx3 antibodies, indicating that these antibodies are specific for Panx1 and Panx3, respectively. Anti -actin or anti-GAPDH was used as a loading control. MW, molecular weight standards; Ventricle, heart ventricle; Panx1, Panx1-expressing NRK cells; Panx3, Panx3-expressing NRK cells.