Fig. 1. RyR subtype expression in WT and RyR1^–/– mouse embryonic detrusor muscle. Total proteins from urinary bladder (100 µg per lane), skeletal muscle (50 µg per lane), cardiac muscle (50 µg per lane) and brain (50 µg per lane) were separated on SDS-PAGE and analyzed by western blot with anti-RyR1, anti-RyR2 and anti-RyR3 antibodies. Skeletal and cardiac muscles were chosen as positive control tissues for the anti-RyR1 and anti-RyR2 antibodies, respectively, and brain extracts were used as positive control for the anti-RyR3 antibody. Similar results were obtained in five independent experiments.