Fig. 5. Intracellular localization of RyR subtypes in WT and RyR1^–/– myocytes. (A-C) Immunostainings performed in WT and RyR1^–/– urinary bladder myocytes with specific antibodies against (A) RyR1, (B) RyR2 and (C) RyR3 and Alexa Fluor 488-coupled secondary antibodies. (Left panels) Transmission images of each immmunostained cell was performed to locate the plasma membrane. The cell outline (dotted) was then reported on the fluorescent confocal image of the cell (middle panel) and fluorescence profiles (a-f) depicting subcellular localization of RyR were drawn for each confocal image (right panel). Edges of cells were reported on fluorescence profiles as dotted lines. Representative pictures of n=10-15 cells in each conditions.