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Figure 6


Fig. 6. Supervillin knockdown decreases the number of peripheral bundles of MHC IIB and disorganizes the peripheral localization of phosphorylated MLC. (A) Localization of F-actin (a,d; green in c,f) and MHC IIB (b,e; red in c,f) in A549 cells treated with hSV1 (a-c) or control (d-f) dsRNAs after spreading on fibronectin for 30 minutes. (B) Localization of activated MLC using antibodies against mono-phosphorylated serine-19 (pS19-MLC; a,d), di-phosphorylated threonine-18, serine-19 (pT18pS19-MLC; b,e) and F-actin visualized with Alexa-Fluor-350-phalloidin (c,f). Cells were treated with hSV2 dsRNA (a-c) or were mock-treated (d-f) before spreading on fibronectin for 30 minutes. Signals were visualized in the red (a,d) and green (b,e) channels. Arrows, peripheral bundles. Bars, 10 µm.