Fig. 8. Inhibition of MLCK mimics the effect of supervillin knockdown on the formation of peripheral bundles of activated myosin during spreading of A549 cells. A549 cells treated with 1 µM ML-7 to specifically inhibit MLCK (a-i) lack the peripheral bundles of activated myosin filaments found in control cells (j-l) after 30 minutes of spreading. Primary antibody staining for MHC IIA (a), vinculin (c,f), MHC IIB (d), pSer19-MLC (g,j) and pThr18pSer19-MLC (h,k) was visualized in the green (a,d,h,j) and red (c,f,g,k) channels. F-actin was visualized with Alexa-Fluor-350–phalloidin (b,e,i,l). Bar, 10 µm.