Fig. 11. Effect of Ika on positively and negatively charged nanogold internalization. (a,b) After 30 minutes incubation with Ika, positively charged nanogold was found in vacuoles (arrows and arrowheads), whereas the Golgi body (b, G) was not labelled. (c) Gold particles in vesicles containing fibrillar cell wall material after 30 minutes of incubation. (d) After 1 hour, positively charged nanogold was seen in small vesicles (black arrowheads). Large vesicles/endosomes were also labelled (asterisks). Golgi bodies in the picture only showed two gold particles (blue arrowheads), whereas one vesicle of the TGN was labelled (yellow arrowhead). (e-g) Negatively charged nanogold was not seen in vacuoles and was only sometimes observed in vesicles (f, arrowhead). Bars, 0.5 µm.