Fig. 3. Internalization of positively charged nanogold for 1-2 hours. (a,b) After 1 hour, vacuoles were labelled (arrows). (c) After 2 hours, gold uptake occurred in subapical regions of the tube (black arrow). (d) Gold particles in vesicles and cisternae of the cis, medial and trans faces of Golgi bodies. (e) A large number of stained vesicles were observed in the tip region. Gold particles were observed in tubules of smooth ER (arrows). Bars, 0.5 µm.