Fig. 2. HASPB-induced plasma membrane blebs are highly dynamic. (A-D) Representative images of N18-HASPB-GFP-induced PM blebs at four different time-points of a fast 2D time-lapse (10 minutes, 150 ms per frame, see also supplementary material Movie 1) show the dynamic appearance and retraction of blebs of different sizes. The kinetics of several blebs was analyzed using kymographs along the axis perpendicular to the bleb base. Boxed areas in C indicate the blebs analyzed in the kymographs shown in E,F. Scale bar, 10 µm. (E,F) Two kymographs (time-space plots; left) along the axis perpendicular to the bleb base and corresponding details of the blebs in the boxed areas in C during formation, retention and retraction (right). Notice the different time intervals between the stills during formation (1 second) and retention as well as retraction (3 seconds).