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Figure 5


Fig. 5. Characterization of SH4-domain-mediated membrane blebbing. (A) PM blebs are dependent on Rock and myosin II activity as well as on an intact microfilament system. CHO cells expressing N18-HASPB-GFP were treated with solvent or the indicated drugs for 3 hours, fixed and stained for F-actin. Shown are representative confocal micrographs. Scale bar, 10 µm. (B) PM blebs depend on an intact microtubule architecture. Cells were treated and processed as described in A, but were additionally stained for {alpha}-tubulin. Scale bar, 10 µm. (C) Quantitative analysis of PM blebbing following drug treatment. CHO N18-HASPB-GFP cells were treated with medium or the indicated drugs for 3 hours (see Materials and Methods for details), fixed, stained for F-actin and evaluated for PM blebbing. Values are the arithmetic means of at least three independent experiments+s.d. In each experiment, over 100 cells were counted per condition. BS, blebbistatin.